Correlative Light & Electron Microscopy, CLEM

Correlative Light & Electron Microscopy (CLEM) is a method to identify a functional protein or tissue by fluorescence microscopy and to observe its morphology by SEM.
A specific functional protein or tissue is subjected to fluorescent staining (labeling). The labeled site is observed and identified using a fluorescence microscope (one of optical microscopes). Then, the fine morphology of the fluorescent-labeled site is observed and analyzed using an SEM with a high spatial resolution. The method is widely used in bio-science.