immunoelectron microscopy
immunoelectron microscopy
Immunoelectron microscopy is a technique to visualize the locations (localization) of specific proteins with a TEM, by utilizing antigen-antibody reactions where antibodies bind specifically to antigenic proteins. First, antibodies (primary antibodies) react with the target proteins. Then, the secondary antibodies labeled with gold colloid, etc., react with the primary antibodies, so that the target proteins can be detected with the TEM. Immunoelectron microscopy is classified into two methods depending on the timing of antibody reactions; pre-embedding (before resin embedding) and post-embedding (after resin embedding).
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