electron staining
electron staining
Electron staining means to adsorb heavy metals of a high scattering power to biological specimens or polymer materials composed of light elements which exhibit a small scattering power for electrons, for enhancing the TEM image contrast of these specimens.
When observing a biological specimen, uranium or lead is adsorbed to proteins, etc., in the specimen. For a specimen of polymers (e.g. polypropylene, polyethylene) consisting of crystalline and amorphous states, ruthenium can be selectively adsorbed to the amorphous regions because ruthenium tetroxide enters only to the amorphous regions. Those heavy metals which strongly scatter incident electrons give rise to enhancement of the image contrast.
When observing a biological specimen, uranium or lead is adsorbed to proteins, etc., in the specimen. For a specimen of polymers (e.g. polypropylene, polyethylene) consisting of crystalline and amorphous states, ruthenium can be selectively adsorbed to the amorphous regions because ruthenium tetroxide enters only to the amorphous regions. Those heavy metals which strongly scatter incident electrons give rise to enhancement of the image contrast.
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