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amorphous ice

amorphous ice

Amorphous ice means ice that does not take a crystalline state. When water is cooled below the melting point, ice crystal is formed and grows until its temperature reaches the re-crystallization temperature.  Since formation of ice crystals can cause destruction of fine structures of a biological specimen, amorphous-ice formation without formation of ice crystals is required when rapid freeze fixation is applied.  Thus, ice-crystal formation should be suppressed by cooling the specimen as rapid as possible down to the re-crystallization temperature.

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