rapid freeze fixation
rapid freeze fixation
Rapid freeze fixation is a technique to physically fix the morphology of biological tissues, biological cells, bacteria and suspended specimens composed of viruses, purified proteins, etc., by rapidly freezing the specimens. Chemical fixation, which is used in ordinary specimen preparation of biological tissues for TEM, may cause outflow of mineral salts, etc., in the tissues or deformation of the membrane structures of the tissues. On the other hand, rapid freeze fixation enables us to fix a specimen while preserving the morphology of the tissues. Rapid freeze fixation techniques include high pressure freezing, metal mirror freezing (slam freezing) and ice embedding.
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