freeze sectioning
freeze sectioning
Freeze sectioning is a method of sectioning by freezing a specimen, which is soft and difficult in preparing an ultrathin section at room temperature, under liquid nitrogen temperature, the specimens specifically including biological specimens without chemical fixation, high-polymers such as rubber.
A cryo-microtome equipped with a cryo-chamber and a liquid-nitrogen Dewar is used for freeze sectioning. Preparation and collection of an ultra-thin section is carried out in a workspace in the cryo-chamber filled with cold gases. In the case of a biological specimen, the specimen does not suffer deformation caused by chemical fixation and dehydration because the section is created from a frozen specimen prepared by rapid freeze fixation. Thus, the method makes possible observation of tissues close to their living state. When observing ultrathin sections with TEM while frozen, a cryo-transfer holder is required.
Liquid nitrogen is automatically supplied to the cryo-chamber to maintain a set temperature.
The workspace is filled with cold gases. The specimen holder moves forward from left to right (along a yellow arrow) by a set value.
A cryo-microtome equipped with a cryo-chamber and a liquid-nitrogen Dewar is used for freeze sectioning. Preparation and collection of an ultra-thin section is carried out in a workspace in the cryo-chamber filled with cold gases. In the case of a biological specimen, the specimen does not suffer deformation caused by chemical fixation and dehydration because the section is created from a frozen specimen prepared by rapid freeze fixation. Thus, the method makes possible observation of tissues close to their living state. When observing ultrathin sections with TEM while frozen, a cryo-transfer holder is required.
Liquid nitrogen is automatically supplied to the cryo-chamber to maintain a set temperature.
The workspace is filled with cold gases. The specimen holder moves forward from left to right (along a yellow arrow) by a set value.
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