Zernike phase contrast
Zernike phase contrast
In TEM, "Zernike phase contrast" means contrast which is obtained by converting the phase change of electron waves scattered by a specimen into the amplitude change. The conversion is performed by using a phase plate or a combined effect of the spherical aberration of the electron lens and defocus.
(a) Conventional TEM (C-TEM) image.
(b) Zernike phase contrast (ZPC-TEM) image of an ice-embedded T4 phage taken at an accelerating voltage of 200 kV.
(c) Schematic of T4 phage.
Compared with the C-TEM image, the ZPC-TEM image clearly visualizes the fine structures of DNA in a capsid, the hair-like structural objects and the cylindrical structures on the capsid surface.
Related Term(s)
Term(s) with "Zernike phase contrast" in the description
Are you a medical professional or personnel engaged in medical care?
No
Please be reminded that these pages are not intended to provide the general public with information about the products.
