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CRYO ARM™ 300 (CRYO ARM™ 300 (JEM-Z300FSC) Field Emission Cryo-Electron MicroscopeJEM-Z300FSC) Field Emission Cryo-Electron Microscope

CRYO ARM™ 300 (JEM-Z300FSC) Field Emission Cryo-Electron Microscope

The JEM-Z300FSC (CRYO ARM™ 300), equipped with a cold field emission gun, an in-column Omega energy filter, a side-entry liquid nitrogen cooling stage and an automated specimen exchange system, is a cryo-electron microscope (cryo-EM) that enables observation of bio-molecules at cryo-temperature. The automated specimen exchange system features the storing of up to 12 samples. In addition, the system allows for the exchange of an arbitrary one or more samples, thus enabling flexible scheduling. Furthermore, the combined use of a newly-designed in-column Omega energy filter and a Hole-free phase plate dramatically enhances the contrast of TEM images of biological specimens.

Features

Automated specimen exchange system

The system is composed of a specimen stage to cool samples to liquid nitrogen temperature and a cryo-transfer system to automatically transfer the cooled samples to the cryo-stage. Liquid nitrogen is automatically supplied to the liquid nitrogen tank as required. This automated system features the storing of up to 12 samples and the exchange of an arbitrary one or more samples while the rest of the samples are kept cooled between the specimen stage and specimen exchange system.

Cold field emission gun (Cold FEG)

A Cold FEG produces a high-brightness electron beam with very small energy spread, offering high coherency. Thus, the CRYO ARM™ 300 achieves high resolution, high contrast imaging.

In-column Omega energy filter

Equipped with an in-column Omega energy filter, the CRYO ARM™ 300 acquires energy filtered images and energy loss spectra. Zero-loss images acquired with the microscope provide high contrast with reduced chromatic aberration.

Automated image acquisition software for Single Particle Analysis

The CRYO ARM™ 300 incorporates automated software. The software allows for automated detection of holes on the specimen grid for efficient acquisition of Single Particle Analysis images.

Hole-free phase plate *1

This unique phase plate is suitable for higher contrast in biological specimens that originally provide only low contrast.

Auto adjustment functions *2

Auto focus, auto coma-free alignment, auto parallel-beam illumination and other automated adjustments are available, enabling image acquisition under optimum conditions.

  • Optional unit.

  • Images acquired with the bottom mount camera are used.

Specifications

Main instrument

Electron gun Cold field emission gun (Cold FEG)
Accelerating voltage 300kV
Energy filter In-column Omega energy filter
Maximum specimen tilt angle ± 70°

Specimen Stage / Automated specimen exchange system

Specimen stage
Coolant Liquid nitrogen
Automated liquid-nitrogen filling system built-in
Specimen cooling temperature 100K or less
Temperature measurement position Specimen, Cryo-shield, LN2 tank
Specimen movements
X、Y Motor drive (movements: ±1 mm)
Piezoelectric elements (movements: ±0.5 μm)
Z Motor drive (movements: ±0.2 mm)
Tilt-X Motor drive (tilts: ±70°)
Rotation within the specimen plane 0° or  90°
Specimen exchange system Air-lock
Automated cryo-transfer system built-in
Cooling temperature
(specimen exchange chamber)
105K or less
Specimen exchange cartridge Up to 4 specimens can be changed at one time.
Specimen parking stage Up to 12 specimens can be held.

Catalogue Download

Application

Application JEM-Z300FSC

Gallery

Reference

Innexin-6 gap junction channel

Experimental conditions

  • Sample: Innexin-6 (Caenorhabditis elegans)

  • Microscope: CRYO ARM™300 (300kV CFEG) with Gatan K2

  • Software used for image acquisition : JADAS (1974 images)

  • Number of particles: 91,613 (Initial pickup), 37,767 (for 3D reconstruction)

  • Software used for image analysis: Relion3

  • Resolution: 3.0 Å (at FSC = 0.143)

Sample by courtesy of Prof. A. Oshima (Nagoya University)

Apoferritin

The highest resolution 1.53 Å achieved by cryoEM 2019.02

mouse apoferritin

  • Optics features: Cold FEG 300 kV & Ω-type energy filter with 20 eV slit width

  • Detector: Gatan K2 (image pixel size: 0.495 Å, mag x100,000)

  • Grid: Quantifoil 1.2/1.3 Cu 200 mesh, kept in the autoloader for 3 days before data collection

  • No. of micrographs: 974 collected over 24 h, 840 used for image analysis

  • No. of particles: 120,295 used for final reconstructionSoftware: RELION 3.1b, CTFFIND4

  • Software: RELION 3.1b, CTFFIND4

  • Resolution: 1.53 Å (B-factor: 47)

  • Note: the first 56 images alone produced a map of 1.76 Å resolution (B-factor: 45)

 

Mouse apoferritin plasmid from Yanagisawa, Danev & Kikkawa @Tokyo University
Kato, Makino, Nakane, Terahara, Kaneko, Shimizu, Motoki, Ishikawa, Yonekura & Namba 2019.02 (EMDB-9865)

ß-galactosidase

β-galactosidase 2.43 Å resolution CRYO ARM™

  • Three-dimensional calibration image of β-galactosidase obtained using a cryo-electron microscope (CRYO ARM™ 200) with an accelerating voltage of 200 kV

  • Sample:
    β-galactosidase with PETG

  • Microscope:
    CRYO ARM™ (Schottky 200 kV) / K2 summit

  • Number of Images:
    2,500 over 3 days by JADAS

  • Image pixel size:
    0.8 Å/pixel

  • Number of particle images:
    350,000(Initial pickup), 88,564 (for final 3D reconstruction)

  • Software:
    Motioncor2, Gctf, Gautomatch, Relion2.0

  • Total dose:
    70 e-/Å2 (70 frames (0.2 sec/frames x 14 sec)

 

Data: courtesy by Dr. T. Kato and Dr. K. Namba, Osaka University, August 2017

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