High Sensitivity Peptide Measurement with the New Matrix alpha-Cyano-4-Chlorocinnamic Acid [MALDI Application]
Matrix assisted laser desorption ionization (MALDI) is a powerful and useful ionization technique that is commonly used for the analysis of biomolecules such as peptides and proteins. Typically, α-Cyano-4-hydroxycinnamic acid (CHCA) is the matrix used for MALDI peptide measurement. Recently, a new matrix “α-Cyano-4-chlorocinnamic acid (CClCA)” was investigated for peptide analysis [1]. In this study, we demonstrate the measurement of a BSA digest to evaluate the improvement in peptide sensitivity with CClCA in comparison with CHCA by using the JMSS3000 SpiralTOF MS system.
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