CRYO ARM™ 300S
Field Emission Cryo-Electron Microscope

One cryo-electron microscope for single-particle analysis, cryo-electron tomography and 3DED/MicroED

AI-driven automation and higher throughput make JEOL cryo-EM faster, more reproducible, and accessible to every operator. JEOL’s latest 300 kV cold field-emission cryo-electron microscope that offers single-particle analysis (SPA), cryo-electron tomography (cryo-ET), 3DED/MicroED and beyond, all in one highly automated instrument. The CRYO ARM™ 300S is engineered to deliver high-resolution structural data with the stability, throughput and ease of use that today’s structural-biology and drug-discovery laboratories require.

Features

CRYO ARM™ 300S × SmartScope
Single particle analysis data acquisition supported by AI

SmartScope ¹ is an open-source software that supports data acquisition in single particle analysis. Using AI, it automatically detects areas suitable for imaging and can automate the entire process up to data acquisition. In addition, with an intuitive UI that organizes the operation flow in a simple manner and with necessary configurations kept to a minimum, even beginners can achieve stable data acquisition regardless of the level of experience.

1. Jonathan Bouvette et al., eLife, 11: e80047 (2022)

Workflow

Interface

Result

Apoferritin Structure with SmartScope

CRYO ARM™ 300S × System Innovations
High throughput, easy operation

Smart sample transfer system CRYO SPECPORTER™-S

The advanced CRYO SPECPORTER™ -S separates the storage for sample preservation from the transfer magazines used for sample exchange. This permits selective handling of only the samples required during analysis, eliminating the need to load and unload all samples in the instrument during exchange. This supports stable and efficient operation, even in multiuser environments.

Enhanced cartridge handling with higher transfer capacity

The number of cartridges that can be loaded into a magazine at one time has expanded from four to six, reducing the frequency and time required for sample exchange, boosting throughput while maintaining stable sample storage environment.

"Endless" multi-grid imaging capability

A flexible sample transfer system allows selective, individual exchange of only the required samples. In multi-grid imaging, samples analyzed can be exchanged as needed while new samples are preloaded in advance, allowing uninterrupted overnight operation and maximizing data acquisition efficiency.

Sample storage in a clean environment

The storage is maintained under vacuum and at liquid nitrogen temperature, enabling sample storage for approximately one month in a clean, ice-accumulation-free state. This allows flexible sample management in accordance with analysis schedules.

Beam designed for maximum information yield

CRYO ARM™ 300S features fringe-free illumination, which prevents interference fringes during imaging. By making efficient use of the beam, data can be acquired with high throughput while minimizing damage to the sample.

Improved stability and flexibility of the electron beam

Improvements to the illumination system, including the condenser lens (CL) and CL aperture, refine the flexibility and stability of the electron beam. Enhanced lens demagnification ratio and aperture positioning accuracy improve beam positioning at the specimen plane. Eight aperture sizes and seven optimized fringe-free beam conditions for each aperture size enable ideal illumination across a wide range of specimens.

Longer consumable life for reduced maintenance

Roots pumps are used for their extended maintenance intervals, reducing downtime and ensuring high system uptime.

CRYO ARM™ 300S × CryoLameller
Cartridge-based cryo-CLEM workflow delivers high efficiency and high reproducibility

Integration with the CryoLameller (CRYO-FIB-SEM) enables a workflow that seamlessly connects sample preparation and analysis. From sample processing to data acquisition, the same cartridge holding the grid can be used throughout the workflow, allowing continuous operation while preserving positional information. With an integrated fluorescence microscope, intuitive and efficient area selection and positioning are achieved using correlative light and electron microscopy (CLEM).

CryoLameller – CRYO ARM™ Workflow

CLEM Application
CLEM imaging of fluorescently labeled mitochondria

Specifications

Main Instrument

Options

• Low accelerating voltage (Please make a separate inquiry about requests for other accelerating voltage except standard)
• STEM unit
• STEM detector (Bright-field/Dark-field)
• Camera system (Direct electron detector and related equipment)
• Smart Operation Software (AERO)
• EDM (Electrostatic Dose Modulator)

Specimen stage/Automated specimen exchange system / Specimen storage

Application

CryoNote

Gallery

CRYO ARM™ 300S × Applications
SPA・Tomography・3DED/MicroED in a single system

Single Particle Analysis

High-resolution three-dimensional structures can be determined by averaging images of numerous identical particles embedded in vitreous ice, even for samples that are difficult to crystallize

Resolution: 2.8-2.9 Å
Cas5-Cas-7-crRNA-CTR-Cas14 quinary complex
Data provided by Dr. Heng Zhang at Tianjin Medical Univ.

CRISPR-Cas system

CRISPR Cas is a genome editing system derived from an adaptive immune mechanism, in which crRNA from CRISPR loci works with Cas enzymes. Among its various types, the type VII system shown on the left is compact and offers the potential for highly precise genome editing. The CRYO ARM™ 300S enables high resolution structural determination, contributing to the elucidation of genome editing mechanisms.

Cas14 with site-specific cleavage activity Structural determination at atomic resolution has elucidated the cleavage mechanism of CTR in type VII CRISPR-Cas systems.

CRISPR-Cas Mechanism

hERG channel (human ether-a-go-go-related gene channel)

The hERG channel is a protein that forms a pathway for potassium ions across the membrane of cardiac muscle cells. Inhibition of its function can lead to life threatening arrhythmias. The figure below presents a high-resolution structure of the hERG channel bound to an anticancer drug, providing critical insights for predicting and mitigating cardiac side effect risks in drug development.

Anticancer Drug-Bound hERG channel
Data provided by Dr. Takeshi Murata and Ryuhi Yoshioka at Chiba Univ.

Tomography

Three-dimensional structures are visualized by reconstructing data acquired while tilting the specimen

Euglena

Euglena is a unicellular organism that exhibits characteristics of both plants and animals and is utilized in applications such as functional foods and biofuels. By observing lamellae prepared with the CryoLameller (CRYO-FIB-SEM) using the CRYO ARM™ 300S, cellular organelles and their structures can be clearly visualized.

The double-membrane structure on the chloroplast surface and thylakoid membranes can be clearly visualized.

Euglena
Sample provided by Dr. Roland Fleck at King's College London

Lipid nanoparticle (LNP)

Visualization of nucleic acids encapsulated in lipid nanoparticles.

LNP including mRNA
Sample provided by Dr. Keisuke Ueda at Chiba Univ.

Lipid nanoparticles are key carriers in drug delivery systems (DDS) and have attracted significant attention as delivery technologies for nucleic acids such as siRNA and mRNA. The CRYO ARM™ 300S enables high resolution visualization of LNP internal structures and nucleic acid localization, offering in-depth analysis for DDS design and optimization.

3DED / MicroED

Atomic-resolution crystal structures are resolved from electron diffraction data obtained from microcrystals

Organic semiconductor

Organic semiconductors are used in applications such as OLED displays in smartphones and solar cells, combining excellent flexibility with processability. The figure on the right shows the structure of a material expected to be used in next-generation transistors, determined from microcrystals using 3DED / MicroED with reduced electron-beam damage.

Ph-anti-benzothieno[5,6-b]benzothieno[3,2-b]thiophene-C10 (antiC10)
Data provided by Dr. Kouji Yonekura at RIKEN, SPring-8
Center / IMRAM, Tohoku Univ.

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