UltraCOOL probe

The detection coil and preamplifier are cooled to cryogenic temperature to reduce thermal noise to an extremely low level, enabling measurement with a sensitivity several times higher than that of conventional room temperature probes. Since this has a lower coil temperature and smaller thermal noise than the SuperCOOL probe circulating system, it can detect signals with the highest sensitivity. The following four types are lined up for the UltraCOOL probe.

・UltraCOOL probe CH type:
The design is optimal for ¹³C and ¹H nuclei making it possible to measure ¹³C with the highest sensitivity.

・UltraCOOL probe TH type:
This enables the measurement of multiple nuclei which is similar to the SuperCOOL probe at the coil temperature of the UltraCOOL probe. The design allows for observations of multiple nucleus of ¹³C and ²⁹Si at a high sensitivity.

・UltraCOOLprobe HX type:
This probe enables the measurement of multiple nuclei which is similar to the SuperCOOL probe at the coil temperature of the UltraCOOL probe.
This probe is optimal for an inverse experiment of ¹H nucleus and ¹H observation.

・UltraCOOL probe HCN type:
A triple resonance NMR measurement is possible for ¹H, ¹³C, and ¹⁵N with this probe.

Features

UltraCOOL probe CH type

The UltraCOOL probe CH type enables ¹³C measurements with the highest sensitivity. Therefore, it is also matched for extremely low-sensitivity experiments such as INADEQUATE. In addition, its stable temperature control enables effective high-temperature analysis, including the characterization of polymer end-group structures.

Notes: For measurements in high temperatures exceeding 100℃, an additional high temperature measurement rotor for the cryogenic probe is required.

High temperature measurement rotor
for cryogenic probe

Instrument: 800 MHz spectrometer equipped with UltraCOOL probe CH type
Sample: 10mg Fisetin in DMSO-d₆
Experiment: INADEQUATE
Number of scans: 192
Experimental time: 1 day

Instrument: 600 MHz spectrometer equipped with UltraCOOL probe CH type
Sample: Polypropylene in ODCB-d₂
Experiment: ¹³C NMR
Number of scans: 512
Temperature: 135℃
Top: Zoomed image of CH₃ group signal bottom edge
Bottom: Whole image

UltraCOOL probe HCN type

The UltraCOOL probe HCN type is a triple resonance NMR probe optimal for ¹H,¹³C, which is capable of simultaneous decoupling of ²H, ¹³C, ¹⁵N, due to four independent channels. This is the most suitable probe for research on proteins, nucleic acids, and metabolome samples.

UltraCOOL probe HCN type

Comparison of sensitivity between the room‑temperature probe and the UltraCOOL probe

Instrument: 600 MHz spectrometer equipped with UltraCOOL probe HCN type
Sample: 0.35 mM ¹³C/ ¹⁵N Ubiquitin (90% H₂O+10% D₂O) Experiment: ¹H-¹⁵N HSQC 1D
Number of scans: 4
Green: UltraCOOL probe HCN type
Blue: HCN triple resonance probe

Example of triple resonance experiments

IInstrument: 700 MHz spectrometer equipped with UltraCOOL probe HCN type
Sample: 0.35 mM ¹³C/ ¹⁵N Ubiquitin (90% H₂O+10% D₂O)
Experiment: 3D CBCA(CO)NH
Data points: 64 (¹³C t₁) ×32 (¹⁵N t₂) ×1024 (¹H t₃) NUS25%
Number of scans: 8
Experimental time: 8 hours

Instrument: 700 MHz spectrometer equipped with UltraCOOL probe HCN type
Sample: 0.35 mM ¹³C/ ¹⁵N Ubiquitin (90% H₂O+10% D₂O)
Experiment: Stripped plot of 3D CBCA(CO)NH and 3D HNCACB, sliced with 15N axis
Data points: 64 (¹³C t₁) ×32 (¹⁵N t₂) ×1024 (¹H t₃) NUS25%
Number of scans: 8
Experimental time: 8 hours per experiment

Example of ¹³C‑detected measurement

Instrument：700MHz spectrometer equipped with UltraCOOL probe HCN type
Sample：0.5mM ¹³C / ¹⁵N GB1 in (90% H₂O / 10% D₂O)
Experiment：
CACO, 40 (t₁) x 512 (t₂) , number of scans:16
CON, 40 (t₁) x 512 (t₂) , number of scans:24
Experimental time： CACO 1.6 hours, CON 2.4 hours

Spectra of unlabeled peptide

Instrument：700MHz spectrometer equipped with UltraCOOL probe HCN type
Sample：4mM Oxytocin(unlabel)
Experiment：
¹³C-HSQC, 128 (t₁) x 1024 (t₂) , number of scans:4
¹⁵N-HSQC, 32 (t₁) x 1024 (t₂) , number of scans:16
Experimental time：each 28 minutes

Probe Arm and Probe Lifter

Cryogenic probes cannot be raised or lowered by hand in a cooled state due to safety concerns, and hence it might be necessary to warm up cryogenic probes to room temperature before manipulating them. However, you can replace the UltraCOOL probe or SuperCOOL probe in the cooled state by using the probe arm or lifter. Also, though the UltraCOOL and SuperCOOL probes are heavier than room temperature probes due to their nature, they can be easily exchanged using the Probe Arm or the Probe Lifter.

Probe Arm

Probe Lifter

Development Secrets

To a New World: Cryogenic probes - The development of “UltraCOOL” & “SuperCOOL” probes