JMS-S3000 SpiralTOF™ series Shared Instrument Applications Notebook

This is an application notebook that summaries a wide variety of applications of the JMS-S3000 SpiralTOF™ series MALDI-TOFMS that are useful as a shared instrument at universities.

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Table of Contents

JMS-S3000 NewSpiralTOF™ MALDI-TOFMS as a Shared Instrument　P1〜

Organic Chemistry　P3〜

• Accurate mass measurement of small molecules: Analysis of medicinal properties in a combination cold remedy by using JMS-S3000 “SpiralTOF™” (MSTips No. 241)
• Accurate mass measurement of a large molecule: Measurement of boroxine cage using JMS-S3000 “SpiralTOF™” (MSTips No. 369)
• Structural analysis of a small molecule: Structural analysis of a small molecule using JMS-S3000 “SpiralTOF™-plus 2.0”ーMS/MS measurement of a photodegradation product of reserpineー (MSTips No. 365)

Polymers / Oligomers / Surfactants　P9〜

• Molecular weight distributions of high molecular weight polymers: Analysis of high molecular weight polystyrene standards by using JMS-S3000 "SpiralTOF™" with Linear TOF option (MSTips No. 199)
• Analysis of anionic surfactants in negative ion detection mode: Structural analysis of anionic surfactants in MALDI negative ion mode using "SpiralTOF™-plus" (MSTips No. 333)
• Analysis of an insoluble polymer with solvent-free method: Analysis of low molecular weight polyethylene with solvent-free method using JMS-S3000 "SpiralTOF™" (MSTips No. 235)

Proteins / Peptides　P15〜

• Molecular weight measurement of proteins: High Sensitivity Analysis of Intact Proteins Using Linear TOF (MSTips No. 227)
• Identification of a protein with peptide mass fingerprinting (PMF) method: Analysis of Bovine Serum Albumin (MSTips No. 166)
• Identification of a protein with MS/MS ion search method: High-Energy CID Analysis of Bovine Serum Albumin (MSTips No. 174)
• Amino acid sequence analysis of a peptide: Structural Analysis of a High Molecular Weight Peptide (MSTips No. 175)
• Amino acid sequence analysis by using In-source Decay (ISD) method: MALDI-ISD Measurements Using Both the SpiralTOF™ Mode and the Linear Mode (MSTips No. 229)

Lipids　P25〜

• Structural analysis of phospholipids: Structural analysis of phospholipids in egg yolk using JMS-S3000 “SpiralTOF™” with TOF-TOF option (MSTips No. 185)

Nucleic Acids　P27〜

• Accurate mass measurement of a synthetic nucleic acid: Analysis of oligonucleotides using JMS-S3000 "SpiralTOF™-plus 2.0" (MSTips No. 364)

Oligosaccharides　P29〜

• Structural analysis of N-linked glycan: Structural analysis of N-linked glycan (MSTips No. 519)

Natural Products　P31〜

• Structural analysis of a natural product: Analysis of the Natural Organic Compound YTX by Using the TOF-TOF Option

Mass Spectrometry Imaging　P33〜

• High mass-resolution MS imaging of lipids in a mouse brain section: Phase analysis of mass spectrometry imaging data using high-resolution MALDI-TOFMS “NewSpiralTOF™” and vertex component analysis (MSTips No. 528)
• High mass-resolution MS imaging of lipids in a mouse brain section: Mass image quality improvement using a machine learning model “FINE-AI Filter” (MSTips No. 526)
• MS imaging of a strawberry fruit: Mass imaging of strawberry using the JMS-S3000 “SpiralTOF™-plus3.0” and transfer plate Poropare™ (MSTips No. 499)

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