Delta Tips: DEPT/INEPT Tool

The DEPT/INEPT Tool is used to extract three sub-spectra of CH, CH₂ and CH₃ signals from a series of DEPT (Distortionless Enhancement by Polarization Transfer) experiments.
The DEPT experiment can be run as either DEPT-45, DEPT-90 and DEPT-135. The number corresponds to a flip angle of the ¹H selection pulse.

The DEPT-45 detects signals of all protonated carbons, i.e. CH, CH₂ and CH₃, with the same phase (sign). The DEPT-90 gives only CH peaks. The DEPT-135 gives signals of all protonated carbons, but CH and CH₃ signals are positive, while CH₂ peaks are negative. The signals of quaternary carbons are absent in all the DEPT spectra. By combining the DEPT-45, DEPT-90 and DEPT-135 spectra, it is possible to determine multiplicity of each carbon signal.

The DEPT/INEPT Tool automatically adds/subtracts DEPT spectra for your convenience.

① Open DEPT-45, DEPT-90 and DEPT-135 data.
② Select Analyze－DEPT/INEPT Analysis to open the DEPT/INEPT Tool window.

③ Click the Select Geometry button in the DEPT/INEPT Tool window.
Note that the cursor has changed into the Finger symbol.
④ Select each DEPT spectrum with the cursor.

⑤ Click the Process button in the DEPT/INEPT Tool window.
The result of DEPT spectra analysis has been shown in a new Data Slate window.

★If you prefer to use your Data Slate window to display the result of the DEPT/INEPT Tool
follow these steps:

⑥ Click the New button in the DEPT/INEPT Tool window.
The cursor has changed into the Finger symbol.
⑦ Click the menu bar in the Data Slate window.
⑧ Click the Process button in the DEPT/INEPT Tool window.
The result of DEPT analysis has been displayed in your selected Data Slate window.

! The intensity of the DEPT signal is sensitive to the J_CH value variation.
If your sample has rather large J_CH Δ, the CH, CH₂ and CH₃ sub-spectra may contain residual signals (artefacts).

Please see the PDF file for the additional information.
Another window opens when you click.

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