Wide Area Observation by Using SiN Window Chip
EM2020-05E
An ultra wide highly precise image (pixels: approx. 5,500 million, pixel size: approx. 17 nm) is obtained by using the automatic montage system "Limitless Panorama (LLP)" function. The combination of a SiN Window Chip with an LLP, enables imaging of the area allowing for observation of the entire embryo, while maintaining the resolution allowing for observation of organelles.
Imaging Condition
| Specimen | Early embryo of drosophila |
| No of images taken | 23 vertical × 8 horizontal (184 in total) |
| Pixels | 39,245 x 14,112 (15% overlap) |
| Imaging field of view | 680 μm × 250 μm |
| Pixel size | 17.2 nm / pixel |
| Imaging time | 40 min |
| Imaging device | JEM-1400Flash (Accelerating voltage : 120 kV) |
Reference
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