NMR software DeltaV5.3.2 Introduction of new features ～"pure shift" (4)～ HSQC

Here we introduce Pure shift HSQC as an example of Pure shift method by using BIRD. Fig.1 shows the Pure shift HSQC which consists of CPMG HSQC¹⁾ and BIRD. And Fig.2 shows expanded spectra of conventional HSQC and Pure shift HSQC. Conventional HSQC shows no splitting along F1 axis, but splitting due to ¹H-¹H couplings along F2 axis. The Pure shift HSQC achieved signal simplification by eliminating ¹H-¹H coupling.
※In these measurements, window function of x axis were set to 1Hz so that original coupling can be confirmed.

Fig.1: Pulse sequence of Pure shift CPMG HSQC

Fig.2: Comparison of HSQC spectra of Sucrose/D₂O

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