3D DOSY (Three Dimensional Diffusion-Ordered NMR Spectroscopy)

DOSY is a method for separating NMR spectra of mixture samples containing  several molecular species by using the difference of diffusion coefficients of the  individual species. The method can be extended to “3D-DOSY”, where the  diffusion coefficient axis is appended to usual 2D spectra, such as COSY, HMQC,  and HMBC. The slice data of the 3D spectra with respect to the diffusion coefficient give the 2D spectra of the individual molecular species.

Measurements for mixture of gluose and α-cyclodextrim

Sample: Glucose 30mg, a-Cyclodextrin 30mg 
Solvent: D₂O 400μl

DOSY-COSY (BPP-STE-DOSY-COSY)

Spectrometer: JNM-ECA500, Measurement time: 15hour 50min, Diffusion parameters: 8=1.2ms, =300ms,
Field gradient: ~0.32T/m,  DOSY process algorithm: SPLMOD

Reference data

Conventional 2D spectra observed for each compound of glucose and α-cyclodextrim

DOSY-HMQC(GC-STE-DOSY-HMQC)

Spectrometer: JNM-ECX400, Measurement time: 51hour 51min, Diffusion parameters: 8=1.2ms, =300ms,
Field gradient: ~0.33T/m,  DOSY process algorithm: SPLMOD

Reference data

DOSY-HMBC (GC-STE-DOSY-HMBC)

Spectrometer: JNM-ECA500, Measurement time: 39hour 7min, Diffusion parameters: 8=1.2ms, =300ms,
Field gradient: ~0.32T/m,  DOSY process algorithm: SPLMOD

Reference data